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Cd33 Car Detection Reagent, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hotairm1 binding to S100A9 protein in MDSCs from septic patients. PBMCs were isolated from early and late septic patients and depleted of the HLA-DR + cells using biotin-coupled anti-HLA-DR antibody and anti-biotin microbeads, followed by the positive selection of <t>CD33</t> + LOX1 + cells with biotin-coupled <t>anti-CD33,</t> anti-CD11b, and anti-LOX1 antibodies. (A) The CD33 + CD11b + LOX1 + HLA-DR- cells were fixed in 0.2% formaldehyde to preserve RNA-protein complexes. The whole cell lysate was incubated with 5 μg of anti-S100A9 or anti-IgG isotype control antibody and then cross-linked to protein G magnetic beads overnight at 4°C. RNA was extracted from the immunoprecipitated protein-RNA complexes by Trizol reagent and used for detecting Hotairm1 by RT-qPCR, using human Hotairm1 primer assay. (B) CD33 + CD11b + LOX1 + HLA-DR- cells from late septic patients were transfected with scramble or Hotairm1 siRNA for 36 h. The cells were treated as described in A , and RT-PCR determined levels of Hotairm1. (C) Whole cell lysate of CD33 + CD11b + LOX1 + HLA-DR-cells were cleared by centrifugation and subjected to immunoprecipitation with S100A9 or IgG isotype control antibody as described in A . The immunoprecipitated protein complexes were assessed by immunoblotting using phospho-S100A9 (Thr113) antibody. The membrane was stripped and reprobed with a phospho-p38 antibody. The results are representative of two experiments. (D) CD33 + CD11b + LOX1 + HLA-DR-cells were transfected with scramble or Hotairm1 siRNA for 36 h, then washed and incubated with 1 μg/ml of bacterial LPS for 12 h. ELISA determined the levels of IL-10 in the culture supernatants. The data are mean ± SD of 5 patients per group.*p < 0.05, vs . early septic or control siRNA; **p < 0.05, vs . Hotairm1 siRNA/early septic; #p < 0.05, vs . control siRNA.
Biotin Coupled Antibodies Against Cd33, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hotairm1 binding to S100A9 protein in MDSCs from septic patients. PBMCs were isolated from early and late septic patients and depleted of the HLA-DR + cells using biotin-coupled anti-HLA-DR antibody and anti-biotin microbeads, followed by the positive selection of <t>CD33</t> + LOX1 + cells with biotin-coupled <t>anti-CD33,</t> anti-CD11b, and anti-LOX1 antibodies. (A) The CD33 + CD11b + LOX1 + HLA-DR- cells were fixed in 0.2% formaldehyde to preserve RNA-protein complexes. The whole cell lysate was incubated with 5 μg of anti-S100A9 or anti-IgG isotype control antibody and then cross-linked to protein G magnetic beads overnight at 4°C. RNA was extracted from the immunoprecipitated protein-RNA complexes by Trizol reagent and used for detecting Hotairm1 by RT-qPCR, using human Hotairm1 primer assay. (B) CD33 + CD11b + LOX1 + HLA-DR- cells from late septic patients were transfected with scramble or Hotairm1 siRNA for 36 h. The cells were treated as described in A , and RT-PCR determined levels of Hotairm1. (C) Whole cell lysate of CD33 + CD11b + LOX1 + HLA-DR-cells were cleared by centrifugation and subjected to immunoprecipitation with S100A9 or IgG isotype control antibody as described in A . The immunoprecipitated protein complexes were assessed by immunoblotting using phospho-S100A9 (Thr113) antibody. The membrane was stripped and reprobed with a phospho-p38 antibody. The results are representative of two experiments. (D) CD33 + CD11b + LOX1 + HLA-DR-cells were transfected with scramble or Hotairm1 siRNA for 36 h, then washed and incubated with 1 μg/ml of bacterial LPS for 12 h. ELISA determined the levels of IL-10 in the culture supernatants. The data are mean ± SD of 5 patients per group.*p < 0.05, vs . early septic or control siRNA; **p < 0.05, vs . Hotairm1 siRNA/early septic; #p < 0.05, vs . control siRNA.
Cd33, supplied by Biorbyt, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd33 antibody, anti-human, biotin  (Miltenyi Biotec)
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Hotairm1 binding to S100A9 protein in MDSCs from septic patients. PBMCs were isolated from early and late septic patients and depleted of the HLA-DR + cells using biotin-coupled anti-HLA-DR antibody and anti-biotin microbeads, followed by the positive selection of <t>CD33</t> + LOX1 + cells with biotin-coupled <t>anti-CD33,</t> anti-CD11b, and anti-LOX1 antibodies. (A) The CD33 + CD11b + LOX1 + HLA-DR- cells were fixed in 0.2% formaldehyde to preserve RNA-protein complexes. The whole cell lysate was incubated with 5 μg of anti-S100A9 or anti-IgG isotype control antibody and then cross-linked to protein G magnetic beads overnight at 4°C. RNA was extracted from the immunoprecipitated protein-RNA complexes by Trizol reagent and used for detecting Hotairm1 by RT-qPCR, using human Hotairm1 primer assay. (B) CD33 + CD11b + LOX1 + HLA-DR- cells from late septic patients were transfected with scramble or Hotairm1 siRNA for 36 h. The cells were treated as described in A , and RT-PCR determined levels of Hotairm1. (C) Whole cell lysate of CD33 + CD11b + LOX1 + HLA-DR-cells were cleared by centrifugation and subjected to immunoprecipitation with S100A9 or IgG isotype control antibody as described in A . The immunoprecipitated protein complexes were assessed by immunoblotting using phospho-S100A9 (Thr113) antibody. The membrane was stripped and reprobed with a phospho-p38 antibody. The results are representative of two experiments. (D) CD33 + CD11b + LOX1 + HLA-DR-cells were transfected with scramble or Hotairm1 siRNA for 36 h, then washed and incubated with 1 μg/ml of bacterial LPS for 12 h. ELISA determined the levels of IL-10 in the culture supernatants. The data are mean ± SD of 5 patients per group.*p < 0.05, vs . early septic or control siRNA; **p < 0.05, vs . Hotairm1 siRNA/early septic; #p < 0.05, vs . control siRNA.
Cd33 Antibody, Anti Human, Biotin, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hotairm1 binding to S100A9 protein in MDSCs from septic patients. PBMCs were isolated from early and late septic patients and depleted of the HLA-DR + cells using biotin-coupled anti-HLA-DR antibody and anti-biotin microbeads, followed by the positive selection of <t>CD33</t> + LOX1 + cells with biotin-coupled <t>anti-CD33,</t> anti-CD11b, and anti-LOX1 antibodies. (A) The CD33 + CD11b + LOX1 + HLA-DR- cells were fixed in 0.2% formaldehyde to preserve RNA-protein complexes. The whole cell lysate was incubated with 5 μg of anti-S100A9 or anti-IgG isotype control antibody and then cross-linked to protein G magnetic beads overnight at 4°C. RNA was extracted from the immunoprecipitated protein-RNA complexes by Trizol reagent and used for detecting Hotairm1 by RT-qPCR, using human Hotairm1 primer assay. (B) CD33 + CD11b + LOX1 + HLA-DR- cells from late septic patients were transfected with scramble or Hotairm1 siRNA for 36 h. The cells were treated as described in A , and RT-PCR determined levels of Hotairm1. (C) Whole cell lysate of CD33 + CD11b + LOX1 + HLA-DR-cells were cleared by centrifugation and subjected to immunoprecipitation with S100A9 or IgG isotype control antibody as described in A . The immunoprecipitated protein complexes were assessed by immunoblotting using phospho-S100A9 (Thr113) antibody. The membrane was stripped and reprobed with a phospho-p38 antibody. The results are representative of two experiments. (D) CD33 + CD11b + LOX1 + HLA-DR-cells were transfected with scramble or Hotairm1 siRNA for 36 h, then washed and incubated with 1 μg/ml of bacterial LPS for 12 h. ELISA determined the levels of IL-10 in the culture supernatants. The data are mean ± SD of 5 patients per group.*p < 0.05, vs . early septic or control siRNA; **p < 0.05, vs . Hotairm1 siRNA/early septic; #p < 0.05, vs . control siRNA.
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anti cd33 antibodies - by Bioz Stars, 2026-03
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biotinylated cd33  (Miltenyi Biotec)
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Hotairm1 binding to S100A9 protein in MDSCs from septic patients. PBMCs were isolated from early and late septic patients and depleted of the HLA-DR + cells using biotin-coupled anti-HLA-DR antibody and anti-biotin microbeads, followed by the positive selection of <t>CD33</t> + LOX1 + cells with biotin-coupled <t>anti-CD33,</t> anti-CD11b, and anti-LOX1 antibodies. (A) The CD33 + CD11b + LOX1 + HLA-DR- cells were fixed in 0.2% formaldehyde to preserve RNA-protein complexes. The whole cell lysate was incubated with 5 μg of anti-S100A9 or anti-IgG isotype control antibody and then cross-linked to protein G magnetic beads overnight at 4°C. RNA was extracted from the immunoprecipitated protein-RNA complexes by Trizol reagent and used for detecting Hotairm1 by RT-qPCR, using human Hotairm1 primer assay. (B) CD33 + CD11b + LOX1 + HLA-DR- cells from late septic patients were transfected with scramble or Hotairm1 siRNA for 36 h. The cells were treated as described in A , and RT-PCR determined levels of Hotairm1. (C) Whole cell lysate of CD33 + CD11b + LOX1 + HLA-DR-cells were cleared by centrifugation and subjected to immunoprecipitation with S100A9 or IgG isotype control antibody as described in A . The immunoprecipitated protein complexes were assessed by immunoblotting using phospho-S100A9 (Thr113) antibody. The membrane was stripped and reprobed with a phospho-p38 antibody. The results are representative of two experiments. (D) CD33 + CD11b + LOX1 + HLA-DR-cells were transfected with scramble or Hotairm1 siRNA for 36 h, then washed and incubated with 1 μg/ml of bacterial LPS for 12 h. ELISA determined the levels of IL-10 in the culture supernatants. The data are mean ± SD of 5 patients per group.*p < 0.05, vs . early septic or control siRNA; **p < 0.05, vs . Hotairm1 siRNA/early septic; #p < 0.05, vs . control siRNA.
Biotinylated Cd33, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd33 car detection reagent biotin  (Miltenyi Biotec)
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Hotairm1 binding to S100A9 protein in MDSCs from septic patients. PBMCs were isolated from early and late septic patients and depleted of the HLA-DR + cells using biotin-coupled anti-HLA-DR antibody and anti-biotin microbeads, followed by the positive selection of <t>CD33</t> + LOX1 + cells with biotin-coupled <t>anti-CD33,</t> anti-CD11b, and anti-LOX1 antibodies. (A) The CD33 + CD11b + LOX1 + HLA-DR- cells were fixed in 0.2% formaldehyde to preserve RNA-protein complexes. The whole cell lysate was incubated with 5 μg of anti-S100A9 or anti-IgG isotype control antibody and then cross-linked to protein G magnetic beads overnight at 4°C. RNA was extracted from the immunoprecipitated protein-RNA complexes by Trizol reagent and used for detecting Hotairm1 by RT-qPCR, using human Hotairm1 primer assay. (B) CD33 + CD11b + LOX1 + HLA-DR- cells from late septic patients were transfected with scramble or Hotairm1 siRNA for 36 h. The cells were treated as described in A , and RT-PCR determined levels of Hotairm1. (C) Whole cell lysate of CD33 + CD11b + LOX1 + HLA-DR-cells were cleared by centrifugation and subjected to immunoprecipitation with S100A9 or IgG isotype control antibody as described in A . The immunoprecipitated protein complexes were assessed by immunoblotting using phospho-S100A9 (Thr113) antibody. The membrane was stripped and reprobed with a phospho-p38 antibody. The results are representative of two experiments. (D) CD33 + CD11b + LOX1 + HLA-DR-cells were transfected with scramble or Hotairm1 siRNA for 36 h, then washed and incubated with 1 μg/ml of bacterial LPS for 12 h. ELISA determined the levels of IL-10 in the culture supernatants. The data are mean ± SD of 5 patients per group.*p < 0.05, vs . early septic or control siRNA; **p < 0.05, vs . Hotairm1 siRNA/early septic; #p < 0.05, vs . control siRNA.
Anti Cd33 Car Detection Reagent Biotin, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd33 car detection reagent biotin - by Bioz Stars, 2026-03
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cd33car detection reagent  (Miltenyi Biotec)
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Miltenyi Biotec cd33car detection reagent
Hotairm1 binding to S100A9 protein in MDSCs from septic patients. PBMCs were isolated from early and late septic patients and depleted of the HLA-DR + cells using biotin-coupled anti-HLA-DR antibody and anti-biotin microbeads, followed by the positive selection of <t>CD33</t> + LOX1 + cells with biotin-coupled <t>anti-CD33,</t> anti-CD11b, and anti-LOX1 antibodies. (A) The CD33 + CD11b + LOX1 + HLA-DR- cells were fixed in 0.2% formaldehyde to preserve RNA-protein complexes. The whole cell lysate was incubated with 5 μg of anti-S100A9 or anti-IgG isotype control antibody and then cross-linked to protein G magnetic beads overnight at 4°C. RNA was extracted from the immunoprecipitated protein-RNA complexes by Trizol reagent and used for detecting Hotairm1 by RT-qPCR, using human Hotairm1 primer assay. (B) CD33 + CD11b + LOX1 + HLA-DR- cells from late septic patients were transfected with scramble or Hotairm1 siRNA for 36 h. The cells were treated as described in A , and RT-PCR determined levels of Hotairm1. (C) Whole cell lysate of CD33 + CD11b + LOX1 + HLA-DR-cells were cleared by centrifugation and subjected to immunoprecipitation with S100A9 or IgG isotype control antibody as described in A . The immunoprecipitated protein complexes were assessed by immunoblotting using phospho-S100A9 (Thr113) antibody. The membrane was stripped and reprobed with a phospho-p38 antibody. The results are representative of two experiments. (D) CD33 + CD11b + LOX1 + HLA-DR-cells were transfected with scramble or Hotairm1 siRNA for 36 h, then washed and incubated with 1 μg/ml of bacterial LPS for 12 h. ELISA determined the levels of IL-10 in the culture supernatants. The data are mean ± SD of 5 patients per group.*p < 0.05, vs . early septic or control siRNA; **p < 0.05, vs . Hotairm1 siRNA/early septic; #p < 0.05, vs . control siRNA.
Cd33car Detection Reagent, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hotairm1 binding to S100A9 protein in MDSCs from septic patients. PBMCs were isolated from early and late septic patients and depleted of the HLA-DR + cells using biotin-coupled anti-HLA-DR antibody and anti-biotin microbeads, followed by the positive selection of CD33 + LOX1 + cells with biotin-coupled anti-CD33, anti-CD11b, and anti-LOX1 antibodies. (A) The CD33 + CD11b + LOX1 + HLA-DR- cells were fixed in 0.2% formaldehyde to preserve RNA-protein complexes. The whole cell lysate was incubated with 5 μg of anti-S100A9 or anti-IgG isotype control antibody and then cross-linked to protein G magnetic beads overnight at 4°C. RNA was extracted from the immunoprecipitated protein-RNA complexes by Trizol reagent and used for detecting Hotairm1 by RT-qPCR, using human Hotairm1 primer assay. (B) CD33 + CD11b + LOX1 + HLA-DR- cells from late septic patients were transfected with scramble or Hotairm1 siRNA for 36 h. The cells were treated as described in A , and RT-PCR determined levels of Hotairm1. (C) Whole cell lysate of CD33 + CD11b + LOX1 + HLA-DR-cells were cleared by centrifugation and subjected to immunoprecipitation with S100A9 or IgG isotype control antibody as described in A . The immunoprecipitated protein complexes were assessed by immunoblotting using phospho-S100A9 (Thr113) antibody. The membrane was stripped and reprobed with a phospho-p38 antibody. The results are representative of two experiments. (D) CD33 + CD11b + LOX1 + HLA-DR-cells were transfected with scramble or Hotairm1 siRNA for 36 h, then washed and incubated with 1 μg/ml of bacterial LPS for 12 h. ELISA determined the levels of IL-10 in the culture supernatants. The data are mean ± SD of 5 patients per group.*p < 0.05, vs . early septic or control siRNA; **p < 0.05, vs . Hotairm1 siRNA/early septic; #p < 0.05, vs . control siRNA.

Journal: Journal of clinical & cellular immunology

Article Title: Hotairm1 Controls S100A9 Protein Phosphorylation in Myeloid-Derived Suppressor Cells during Sepsis

doi:

Figure Lengend Snippet: Hotairm1 binding to S100A9 protein in MDSCs from septic patients. PBMCs were isolated from early and late septic patients and depleted of the HLA-DR + cells using biotin-coupled anti-HLA-DR antibody and anti-biotin microbeads, followed by the positive selection of CD33 + LOX1 + cells with biotin-coupled anti-CD33, anti-CD11b, and anti-LOX1 antibodies. (A) The CD33 + CD11b + LOX1 + HLA-DR- cells were fixed in 0.2% formaldehyde to preserve RNA-protein complexes. The whole cell lysate was incubated with 5 μg of anti-S100A9 or anti-IgG isotype control antibody and then cross-linked to protein G magnetic beads overnight at 4°C. RNA was extracted from the immunoprecipitated protein-RNA complexes by Trizol reagent and used for detecting Hotairm1 by RT-qPCR, using human Hotairm1 primer assay. (B) CD33 + CD11b + LOX1 + HLA-DR- cells from late septic patients were transfected with scramble or Hotairm1 siRNA for 36 h. The cells were treated as described in A , and RT-PCR determined levels of Hotairm1. (C) Whole cell lysate of CD33 + CD11b + LOX1 + HLA-DR-cells were cleared by centrifugation and subjected to immunoprecipitation with S100A9 or IgG isotype control antibody as described in A . The immunoprecipitated protein complexes were assessed by immunoblotting using phospho-S100A9 (Thr113) antibody. The membrane was stripped and reprobed with a phospho-p38 antibody. The results are representative of two experiments. (D) CD33 + CD11b + LOX1 + HLA-DR-cells were transfected with scramble or Hotairm1 siRNA for 36 h, then washed and incubated with 1 μg/ml of bacterial LPS for 12 h. ELISA determined the levels of IL-10 in the culture supernatants. The data are mean ± SD of 5 patients per group.*p < 0.05, vs . early septic or control siRNA; **p < 0.05, vs . Hotairm1 siRNA/early septic; #p < 0.05, vs . control siRNA.

Article Snippet: Next, the remaining cells were subjected to positive selection using biotin-coupled antibodies against CD33 (Cat #MA1-19522; Invitrogen, Waltham, MA), CD11b (Cat #130-113-795), and LOX-1 (Cat #130-122-119; both from Milteny Biotec).

Techniques: Binding Assay, Isolation, Selection, Incubation, Control, Magnetic Beads, Immunoprecipitation, Quantitative RT-PCR, Transfection, Reverse Transcription Polymerase Chain Reaction, Centrifugation, Western Blot, Membrane, Enzyme-linked Immunosorbent Assay